Analysis of gallic acid and ellagic acid in leaves of Elaeagnus angustifolia L. from different habitats and times in Xinjiang by HPLC with cluster analysis

Abstract Objective To determine the levels of gallic acid and ellagic by using high-performance liquid chromatography (HPLC) with R software hierarchical cluster analysisin Elaeagnus angustifolia L. gathered from different locations in Xinjiang. Methods A chromatographic column Diamonsil C18 a size 4.6 × 250 mm 5 ?m was used methanol as 0.1% phosphoric aqueous solution B mobile phase. The flow rate 1 mL/min for gradient elution injection volume ?L. HPLC performed detection wavelength 260 nm 35 °C. In addition, clustering method studying E. 10 areas. Results Gallic showed good linear relationship between 7.375 236 ?g/mL correlation coefficient 0.9999, 3.625 116 0.9999 respectively. average recovery values were 103.98 101.57%, Relative Standard Deviation (RSD) 1.92 1.47%. Conclusion Differences leaves areas Xinjiang that both highest Kuitun.